Finding intermodel H-bonds Finding intramodel H-bonds Constraints relaxed by 0.4 angstroms and 20 degrees Models used: #0 2afx_A.cif H-bonds (donor, acceptor, hydrogen, D..A dist, D-H..A dist): TRP 4.A N SER 2.A OG no hydrogen 3.085 N/A GLU 6.A N ALA 3.A O no hydrogen 2.842 N/A GLU 7.A N TRP 4.A O no hydrogen 2.943 N/A LYS 8.A NZ PRO 5.A O no hydrogen 2.729 N/A LYS 8.A NZ HIS 201.A O no hydrogen 3.022 N/A ASN 9.A N GLU 6.A O no hydrogen 2.946 N/A TYR 10.A N GLU 7.A O no hydrogen 2.996 N/A GLN 12.A NE2 TYR 10.A O no hydrogen 3.224 N/A ALA 14.A N GLU 319.A O no hydrogen 2.954 N/A LEU 16.A N GLU 319.A OE2 no hydrogen 2.807 N/A ALA 20.A N ASN 17.A OD1 no hydrogen 2.925 N/A LEU 21.A N ASN 17.A O no hydrogen 2.897 N/A ARG 22.A N SER 18.A O no hydrogen 2.886 N/A GLN 23.A N SER 19.A O no hydrogen 2.972 N/A ILE 24.A N ALA 20.A O no hydrogen 2.920 N/A ALA 25.A N LEU 21.A O no hydrogen 2.981 N/A GLU 26.A N ARG 22.A O no hydrogen 3.011 N/A GLY 27.A N ILE 24.A O no hydrogen 3.027 N/A THR 28.A OG1 GLU 137.A OE2 no hydrogen 2.585 N/A SER 29.A N GLU 137.A OE2 no hydrogen 2.996 N/A SER 31.A OG SER 29.A OG no hydrogen 3.201 N/A GLU 32.A N SER 29.A OG no hydrogen 3.064 N/A MET 33.A N SER 29.A O no hydrogen 2.975 N/A TRP 34.A N ILE 30.A O no hydrogen 2.904 N/A TRP 34.A NE1 GLU 298.A OE2 no hydrogen 2.781 N/A GLN 35.A N SER 31.A O no hydrogen 3.045 N/A GLN 35.A NE2 ASN 36.A OD1 no hydrogen 3.317 N/A ASN 36.A N GLU 32.A O no hydrogen 2.826 N/A ASP 37.A N MET 33.A O no hydrogen 2.808 N/A LEU 38.A N MET 33.A O no hydrogen 2.879 N/A GLN 39.A N TRP 34.A O no hydrogen 2.986 N/A GLN 39.A NE2 GLU 298.A OE2 no hydrogen 2.927 N/A LEU 41.A N LEU 38.A O no hydrogen 2.765 N/A LEU 42.A N GLN 39.A O no hydrogen 3.094 N/A ILE 43.A N LEU 41.A O no hydrogen 2.763 N/A ARG 45.A N SER 111.A OG no hydrogen 2.745 N/A ARG 45.A NE TYR 109.A O no hydrogen 2.819 N/A ARG 45.A NH1 ILE 43.A O no hydrogen 2.832 N/A ARG 45.A NH1 VAL 123.A O no hydrogen 2.702 N/A ARG 45.A NH2 VAL 123.A O no hydrogen 3.246 N/A ARG 45.A NH2 GLY 124.A O no hydrogen 3.258 N/A SER 49.A N TYR 46.A O no hydrogen 3.052 N/A SER 49.A OG TYR 46.A O no hydrogen 2.614 N/A SER 52.A N SER 49.A O no hydrogen 3.000 N/A SER 52.A N SER 49.A OG no hydrogen 3.061 N/A TYR 53.A N SER 49.A O no hydrogen 3.303 N/A ALA 54.A N PRO 50.A O no hydrogen 2.977 N/A ALA 55.A N GLY 51.A O no hydrogen 2.820 N/A ARG 56.A N SER 52.A O no hydrogen 2.939 N/A ARG 56.A NH1 SER 52.A OG no hydrogen 3.186 N/A ARG 56.A NH1 TYR 109.A OH no hydrogen 2.876 N/A GLN 57.A N TYR 53.A O no hydrogen 2.940 N/A HIS 58.A N ALA 54.A O no hydrogen 2.943 N/A HIS 58.A NE2 ASP 37.A O no hydrogen 2.841 N/A ILE 59.A N ALA 55.A O no hydrogen 2.903 N/A MET 60.A N ARG 56.A O no hydrogen 2.958 N/A GLN 61.A N GLN 57.A O no hydrogen 2.853 N/A ARG 62.A N HIS 58.A O no hydrogen 2.942 N/A ARG 62.A NE ASP 37.A OD2 no hydrogen 2.673 N/A ARG 62.A NH2 ASP 37.A OD1 no hydrogen 3.191 N/A ARG 62.A NH2 ASP 37.A OD2 no hydrogen 3.465 N/A ILE 63.A N ILE 59.A O no hydrogen 3.138 N/A GLN 64.A N MET 60.A O no hydrogen 2.853 N/A ARG 65.A N GLN 61.A O no hydrogen 3.151 N/A LEU 66.A N ILE 63.A O no hydrogen 3.064 N/A GLN 67.A N ASP 143.A OD2 no hydrogen 2.785 N/A ALA 68.A N ASP 143.A OD1 no hydrogen 2.885 N/A TRP 70.A N ALA 68.A O no hydrogen 2.893 N/A VAL 71.A N THR 94.A O no hydrogen 2.875 N/A GLU 73.A N ILE 92.A O no hydrogen 2.899 N/A ASP 75.A N ASN 90.A O no hydrogen 2.895 N/A THR 76.A OG1 SER 89.A OG no hydrogen 2.654 N/A PHE 77.A N PHE 88.A O no hydrogen 2.913 N/A SER 79.A N ARG 86.A O no hydrogen 2.950 N/A SER 79.A OG GLN 80.A O no hydrogen 3.029 N/A THR 81.A OG1 GLY 84.A O no hydrogen 2.599 N/A TYR 83.A N THR 81.A OG1 no hydrogen 2.961 N/A GLY 84.A N THR 81.A O no hydrogen 2.871 N/A ARG 86.A N SER 79.A O no hydrogen 2.974 N/A ARG 86.A NH2 PRO 47.A O no hydrogen 2.812 N/A PHE 88.A N PHE 77.A O no hydrogen 2.878 N/A SER 89.A N ASP 161.A OD2 no hydrogen 2.904 N/A SER 89.A OG THR 76.A OG1 no hydrogen 2.654 N/A ASN 90.A N ASP 75.A O no hydrogen 2.816 N/A ASN 90.A ND2 ASP 75.A OD2 no hydrogen 2.854 N/A ASN 90.A ND2 GLY 177.A O no hydrogen 2.991 N/A ILE 91.A N PHE 159.A O no hydrogen 2.933 N/A ILE 92.A N GLU 73.A O no hydrogen 2.941 N/A SER 93.A N LEU 157.A O no hydrogen 2.863 N/A THR 94.A N VAL 71.A O no hydrogen 2.885 N/A THR 94.A OG1 GLN 156.A OE1 no hydrogen 2.901 N/A LEU 95.A N LEU 155.A O no hydrogen 2.938 N/A ASN 96.A ND2 LEU 149.A O no hydrogen 3.581 N/A ASN 96.A ND2 SER 154.A OG no hydrogen 2.698 N/A ALA 99.A N ASN 96.A O no hydrogen 2.878 N/A LYS 100.A N ASP 152.A OD2 no hydrogen 2.880 N/A LYS 100.A NZ ASP 152.A OD2 no hydrogen 2.910 N/A ARG 101.A N ASP 152.A O no hydrogen 3.048 N/A ARG 101.A NE ASP 204.A OD1 no hydrogen 2.861 N/A ARG 101.A NE ASP 204.A OD2 no hydrogen 3.406 N/A ARG 101.A NH1 GLU 7.A OE1 no hydrogen 3.018 N/A ARG 101.A NH2 ASP 204.A OD2 no hydrogen 2.780 N/A HIS 102.A N GLY 202.A O no hydrogen 2.893 N/A HIS 102.A NE2 LEU 95.A O no hydrogen 2.801 N/A LEU 103.A N SER 154.A O no hydrogen 2.888 N/A VAL 104.A N LEU 205.A O no hydrogen 2.986 N/A LEU 105.A N GLN 156.A O no hydrogen 2.967 N/A ALA 106.A N VAL 207.A O no hydrogen 2.899 N/A CYS 107.A N ILE 158.A O no hydrogen 3.121 N/A CYS 107.A SG HIS 108.A O no hydrogen 3.857 N/A HIS 108.A ND1 ASP 110.A OD1 no hydrogen 2.755 N/A HIS 108.A NE2 ASP 127.A OD1 no hydrogen 2.742 N/A TYR 109.A N PHE 160.A O no hydrogen 2.792 N/A TYR 109.A OH SER 89.A O no hydrogen 2.630 N/A SER 111.A N ARG 45.A O no hydrogen 3.193 N/A LYS 112.A NZ ALA 165.A O no hydrogen 3.141 N/A LYS 112.A NZ LEU 167.A O no hydrogen 2.884 N/A TRP 117.A N ARG 120.A O no hydrogen 3.222 N/A ARG 120.A N TRP 117.A O no hydrogen 2.999 N/A ARG 120.A NH1 ASP 295.A OD1 no hydrogen 3.391 N/A ARG 120.A NH2 ASP 295.A OD1 no hydrogen 2.869 N/A ALA 125.A N ASP 296.A O no hydrogen 3.050 N/A THR 126.A N ASP 296.A OD1 no hydrogen 2.905 N/A THR 126.A OG1 ASP 296.A OD1 no hydrogen 3.169 N/A THR 126.A OG1 ASP 296.A OD2 no hydrogen 3.007 N/A ASP 127.A N GLY 124.A O no hydrogen 3.061 N/A ALA 129.A N GLY 124.A O no hydrogen 3.165 N/A VAL 130.A N ALA 125.A O no hydrogen 2.812 N/A CYS 132.A N SER 128.A O no hydrogen 2.984 N/A CYS 132.A SG HIS 108.A O no hydrogen 3.763 N/A CYS 132.A SG SER 128.A O no hydrogen 3.464 N/A ALA 133.A N ALA 129.A O no hydrogen 2.983 N/A MET 134.A N VAL 130.A O no hydrogen 2.804 N/A MET 135.A N PRO 131.A O no hydrogen 3.004 N/A LEU 136.A N CYS 132.A O no hydrogen 3.035 N/A GLU 137.A N ALA 133.A O no hydrogen 2.792 N/A LEU 138.A N MET 134.A O no hydrogen 2.908 N/A ALA 139.A N MET 135.A O no hydrogen 3.028 N/A ARG 140.A N LEU 136.A O no hydrogen 2.970 N/A ARG 140.A NE GLU 137.A OE1 no hydrogen 2.905 N/A ARG 140.A NH2 GLY 27.A O no hydrogen 3.335 N/A ARG 140.A NH2 GLU 137.A OE1 no hydrogen 3.493 N/A ARG 140.A NH2 GLU 137.A OE2 no hydrogen 2.924 N/A ALA 141.A N GLU 137.A O no hydrogen 2.718 N/A LEU 142.A N LEU 138.A O no hydrogen 2.964 N/A ASP 143.A N ARG 140.A O no hydrogen 3.127 N/A LYS 145.A NZ LEU 323.A O no hydrogen 3.106 N/A LEU 146.A N LEU 142.A O no hydrogen 2.778 N/A LEU 147.A N ASP 143.A O no hydrogen 2.770 N/A SER 148.A N LYS 145.A O no hydrogen 3.139 N/A SER 148.A OG LYS 145.A O no hydrogen 2.722 N/A LEU 149.A N LEU 146.A O no hydrogen 3.008 N/A LYS 150.A N LEU 147.A O no hydrogen 3.160 N/A LEU 153.A N PRO 151.A O no hydrogen 2.740 N/A SER 154.A N ARG 101.A O no hydrogen 2.965 N/A SER 154.A OG LEU 95.A O no hydrogen 3.256 N/A GLN 156.A N LEU 103.A O no hydrogen 2.932 N/A LEU 157.A N SER 93.A O no hydrogen 2.758 N/A ILE 158.A N LEU 105.A O no hydrogen 2.865 N/A PHE 159.A N ILE 91.A O no hydrogen 2.853 N/A PHE 160.A N CYS 107.A O no hydrogen 2.807 N/A ASP 161.A N ASN 90.A OD1 no hydrogen 2.951 N/A GLY 162.A N ASP 161.A OD1 no hydrogen 2.786 N/A GLU 163.A N ASP 110.A OD1 no hydrogen 3.106 N/A GLU 164.A N ASP 110.A OD2 no hydrogen 3.148 N/A PHE 166.A N ASP 173.A OD2 no hydrogen 2.798 N/A LEU 167.A N ASP 173.A OD1 no hydrogen 2.866 N/A SER 170.A N ASP 173.A O no hydrogen 3.444 N/A ASP 173.A N SER 170.A O no hydrogen 3.291 N/A ASP 173.A N SER 170.A OG no hydrogen 3.223 N/A SER 174.A OG SER 170.A O no hydrogen 2.903 N/A LEU 175.A N GLU 163.A O no hydrogen 2.920 N/A TYR 176.A N GLY 162.A O no hydrogen 2.862 N/A TYR 176.A OH ASP 173.A OD2 no hydrogen 2.644 N/A GLY 177.A N ASP 161.A O no hydrogen 2.998 N/A SER 178.A OG LEU 175.A O no hydrogen 3.512 N/A SER 178.A OG ASP 268.A OD1 no hydrogen 2.710 N/A ARG 179.A N LEU 175.A O no hydrogen 2.942 N/A ARG 179.A NE SER 174.A O no hydrogen 3.068 N/A ARG 179.A NH2 SER 174.A O no hydrogen 2.936 N/A HIS 180.A N TYR 176.A O no hydrogen 3.183 N/A LEU 181.A N GLY 177.A O no hydrogen 2.877 N/A ALA 182.A N SER 178.A O no hydrogen 2.890 N/A ALA 183.A N ARG 179.A O no hydrogen 3.256 N/A LYS 184.A N HIS 180.A O no hydrogen 2.987 N/A LYS 184.A NZ ASP 75.A OD1 no hydrogen 3.306 N/A MET 185.A N LEU 181.A O no hydrogen 2.756 N/A ALA 186.A N ALA 182.A O no hydrogen 2.921 N/A SER 187.A N ALA 183.A O no hydrogen 3.200 N/A SER 187.A N LYS 184.A O no hydrogen 3.171 N/A SER 187.A OG ALA 183.A O no hydrogen 3.038 N/A SER 187.A OG LYS 184.A O no hydrogen 3.208 N/A THR 188.A N MET 185.A O no hydrogen 3.216 N/A THR 188.A OG1 LYS 184.A O no hydrogen 2.694 N/A HIS 190.A N THR 197.A O no hydrogen 2.935 N/A HIS 190.A NE2 ALA 99.A O no hydrogen 2.764 N/A ALA 194.A N PRO 191.A O no hydrogen 2.948 N/A THR 197.A OG1 HIS 190.A O no hydrogen 2.612 N/A SER 198.A N HIS 201.A ND1 no hydrogen 2.890 N/A GLN 199.A N THR 188.A O no hydrogen 2.965 N/A LEU 200.A N SER 198.A OG no hydrogen 2.952 N/A HIS 201.A N SER 198.A OG no hydrogen 3.374 N/A GLY 202.A N SER 198.A O no hydrogen 3.323 N/A MET 203.A N LEU 200.A O no hydrogen 3.011 N/A ASP 204.A N HIS 102.A O no hydrogen 2.895 N/A LEU 206.A N PRO 278.A O no hydrogen 2.901 N/A VAL 207.A N VAL 104.A O no hydrogen 2.764 N/A LEU 208.A N LEU 280.A O no hydrogen 2.972 N/A LEU 209.A N ALA 106.A O no hydrogen 2.868 N/A ASP 210.A N LEU 282.A O no hydrogen 3.069 N/A ILE 212.A N THR 126.A O no hydrogen 3.186 N/A THR 218.A OG1 TYR 259.A OH no hydrogen 3.198 N/A THR 218.A OG1 SER 285.A O no hydrogen 2.701 N/A PHE 219.A N TYR 255.A O no hydrogen 2.855 N/A ASN 221.A N TYR 259.A O no hydrogen 2.889 N/A SER 226.A N PHE 223.A O no hydrogen 3.096 N/A SER 226.A OG PHE 223.A O no hydrogen 3.473 N/A SER 226.A OG VAL 279.A O no hydrogen 2.691 N/A ALA 227.A N PRO 224.A O no hydrogen 3.357 N/A ARG 228.A NH1 HIS 11.A O no hydrogen 2.909 N/A ARG 228.A NH2 LYS 8.A O no hydrogen 3.110 N/A ARG 228.A NH2 HIS 11.A O no hydrogen 2.947 N/A TRP 229.A N SER 226.A O no hydrogen 2.837 N/A PHE 230.A N SER 226.A O no hydrogen 3.006 N/A GLU 231.A N ALA 227.A O no hydrogen 2.896 N/A ARG 232.A N ARG 228.A O no hydrogen 3.243 N/A ARG 232.A NH2 GLU 319.A OE1 no hydrogen 2.812 N/A LEU 233.A N TRP 229.A O no hydrogen 3.104 N/A GLN 234.A N PHE 230.A O no hydrogen 2.977 N/A ALA 235.A N GLU 231.A O no hydrogen 2.865 N/A ILE 236.A N ARG 232.A O no hydrogen 2.821 N/A GLU 237.A N LEU 233.A O no hydrogen 2.914 N/A HIS 238.A N GLN 234.A O no hydrogen 2.957 N/A GLU 239.A N ALA 235.A O no hydrogen 2.891 N/A LEU 240.A N ILE 236.A O no hydrogen 2.891 N/A HIS 241.A N GLU 237.A O no hydrogen 2.909 N/A HIS 241.A ND1 GLU 242.A OE2 no hydrogen 3.274 N/A GLU 242.A N HIS 238.A O no hydrogen 2.860 N/A LEU 243.A N GLU 239.A O no hydrogen 2.976 N/A GLY 244.A N HIS 241.A O no hydrogen 2.906 N/A LEU 245.A N LEU 240.A O no hydrogen 2.957 N/A LYS 247.A N ASN 308.A OD1 no hydrogen 2.859 N/A HIS 249.A NE2 GLU 237.A OE2 no hydrogen 2.790 N/A GLY 253.A N SER 250.A OG no hydrogen 3.096 N/A ARG 254.A N LEU 251.A O no hydrogen 3.114 N/A ARG 254.A NH1 LEU 251.A O no hydrogen 2.877 N/A ARG 254.A NH2 ASN 258.A OD1 no hydrogen 3.400 N/A TYR 255.A N GLU 237.A OE2 no hydrogen 2.770 N/A PHE 256.A N GLU 237.A OE1 no hydrogen 3.185 N/A GLN 257.A NE2 ARG 254.A O no hydrogen 3.050 N/A TYR 259.A OH THR 218.A O no hydrogen 3.234 N/A TYR 259.A OH THR 218.A OG1 no hydrogen 3.198 N/A TYR 259.A OH SER 285.A O no hydrogen 2.935 N/A ASP 268.A N ASP 267.A OD1 no hydrogen 2.743 N/A ILE 270.A N ASP 267.A O no hydrogen 3.000 N/A PHE 272.A N HIS 269.A O no hydrogen 3.010 N/A LEU 273.A N HIS 269.A O no hydrogen 2.739 N/A ARG 274.A N ILE 270.A O no hydrogen 2.896 N/A ARG 275.A N PHE 272.A O no hydrogen 2.941 N/A ARG 275.A NH1 PRO 271.A O no hydrogen 2.883 N/A GLY 276.A N LEU 273.A O no hydrogen 2.925 N/A VAL 277.A N PHE 272.A O no hydrogen 3.073 N/A LEU 280.A N LEU 206.A O no hydrogen 2.850 N/A HIS 281.A NE2 ASP 267.A OD2 no hydrogen 2.869 N/A LEU 282.A N LEU 208.A O no hydrogen 2.757 N/A SER 285.A N THR 218.A O no hydrogen 2.886 N/A TRP 291.A N PRO 288.A O no hydrogen 3.002 N/A THR 293.A N VAL 290.A O no hydrogen 3.066 N/A ASP 296.A N THR 293.A O no hydrogen 2.908 N/A ASN 297.A N ASP 295.A O no hydrogen 2.743 N/A ASN 300.A N ASN 297.A OD1 no hydrogen 3.114 N/A LEU 301.A N GLU 298.A O no hydrogen 3.093 N/A ASP 302.A N GLY 213.A O no hydrogen 2.794 N/A SER 304.A OG ASP 302.A OD1 no hydrogen 3.104 N/A THR 305.A N ASP 302.A OD1 no hydrogen 3.083 N/A ILE 306.A N ASP 302.A O no hydrogen 3.187 N/A ASP 307.A N GLU 303.A O no hydrogen 2.931 N/A ASN 308.A N SER 304.A O no hydrogen 2.864 N/A ASN 308.A ND2 LYS 247.A O no hydrogen 2.838 N/A ASN 308.A ND2 TYR 255.A OH no hydrogen 3.036 N/A LEU 309.A N THR 305.A O no hydrogen 2.923 N/A ASN 310.A N ILE 306.A O no hydrogen 2.808 N/A LYS 311.A N ASP 307.A O no hydrogen 3.104 N/A LYS 311.A NZ LEU 245.A O no hydrogen 2.818 N/A ILE 312.A N ASN 308.A O no hydrogen 2.988 N/A LEU 313.A N LEU 309.A O no hydrogen 2.875 N/A GLN 314.A N ASN 310.A O no hydrogen 2.871 N/A GLN 314.A NE2 THR 28.A OG1 no hydrogen 3.202 N/A VAL 315.A N LYS 311.A O no hydrogen 3.083 N/A PHE 316.A N ILE 312.A O no hydrogen 2.991 N/A VAL 317.A N LEU 313.A O no hydrogen 2.947 N/A LEU 318.A N GLN 314.A O no hydrogen 2.991 N/A GLU 319.A N VAL 315.A O no hydrogen 2.882 N/A TYR 320.A N PHE 316.A O no hydrogen 2.926 N/A TYR 320.A OH ASP 204.A OD2 no hydrogen 2.515 N/A LEU 321.A N VAL 317.A O no hydrogen 2.941 N/A HIS 322.A N GLU 319.A O no hydrogen 3.425 N/A LEU 323.A N LEU 318.A O no hydrogen 2.906 N/A